Review




Structured Review

Proteintech ythdc2
Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of <t>YTHDC2</t> , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.
Ythdc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ythdc2+antibody/YTHDC2+Antibody/pmc12862939-61-52-53
Average 95 stars, based on 50 article reviews
ythdc2 - by Bioz Stars, 2026-10
95/100 stars

Images

1) Product Images from "METTL14 alleviates heat stress in Hu sheep involves enhancing fatty acid oxidation while reducing lipid deposition"

Article Title: METTL14 alleviates heat stress in Hu sheep involves enhancing fatty acid oxidation while reducing lipid deposition

Journal: Frontiers in Veterinary Science

doi: 10.3389/fvets.2025.1732947

Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.
Figure Legend Snippet: Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

Techniques Used: Expressing, Methylation, Immunofluorescence, RNA Sequencing

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Incubation:

Article Title: Mettl3 promotes oxLDL‐mediated inflammation through activating STAT1 signaling
Article Snippet: Protein extractions were separated by 10% SDS‐PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. After the incubation with primary antibodies, including anti‐Mettl3 antibody (1:1000, Abcam, USA), anti‐Mettl14 antibody (1:1000, Abcam, USA), anti‐WTAP antibody (1:1000, Abcam, USA), anti‐flag antibody (1:1000, Abcam, USA), anti‐phosphorylated p38 antibody (p‐p38, 1:1000, Abcam, USA), anti‐p38 antibody (1:1000, Proteintech, China), anti‐phosphorylated JNK antibody (p‐JNK, 1:1000, Abcam, USA), anti‐JNK antibody (1:1000, Proteintech, China), anti‐phosphorylated p65 antibody (p‐p65, 1:1000, Abcam, USA), anti‐p65 antibody (1:1000, Proteintech, China), anti‐phosphorylated STAT1 antibody (p‐STAT1, 1:1000, Abcam, USA), anti‐STAT1 antibody (1:1000, Proteintech, China), anti‐phosphorylated STAT3 antibody (p‐STAT3, 1:1000, Abcam, USA), anti‐STAT3 antibody (1:1000, Proteintech, China), anti‐LOX‐1 antibody (1:1000, Proteintech, China), anti‐FTO antibody (1:1000, Proteintech, China), anti‐ALKBH5 antibody (1:1000, Proteintech, China), anti‐RBM15 antibody (1:1000, Proteintech, China), anti‐YTHDF1 antibody (1:1000, Proteintech, China), anti‐YTHDF2 antibody (1:1000, Proteintech, China), anti‐YTHDF3 antibody (1:1000, Proteintech, China), anti‐IGF2BP1 antibody (1:1000, Proteintech, China), anti‐IGF2BP2 antibody (1:1000, Proteintech, China), anti‐IGF2BP3 antibody (1:1000, Proteintech, China), anti‐EIF3A antibody (1:1000, Proteintech, China), anti‐YTHDC1 antibody (1:1000, Proteintech, China), anti‐YTHDC2 antibody (1:1000, Proteintech, China), and anti‐GAPDH antibody (1:1000, Proteintech, China), the membranes were then incubated with peroxidase (HRP)‐conjugated secondary antibody. .. After washes with PBS, protein bands were detected using a chemiluminescence system (Bio‐Rad, USA) and analyzed using Image Lab Software.

Article Title: Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling
Article Snippet: Protein extractions were separated by 10% SDSPAGE and transferred onto polyvinylidene uoride (PVDF) membranes (Millipore, USA). .. After the incubation with primary antibodies, including anti-Mettl3 antibody (1:1000, Abcam, USA), anti-Mettl14 antibody (1:1000, Abcam, USA), anti-WTAP antibody (1:1000, Abcam, USA), anti- ag antibody (1:1000, Abcam, USA), anti-phosphorylated p38 antibody (p-p38, 1:1000, Abcam, USA), anti-p38 antibody (1:1000, Proteintech, China), anti-phosphorylated JNK antibody (p-JNK, 1:1000, Abcam, USA), anti-JNK antibody (1:1000, Proteintech, China), anti-phosphorylated p65 antibody (p-p65, 1:1000, Abcam, USA), anti-p65 antibody (1:1000, Proteintech, China), anti-phosphorylated STAT1 antibody (p-STAT1, 1:1000, Abcam, USA), anti-STAT1 antibody (1:1000, Proteintech, China), anti-phosphorylated STAT3 antibody (p-STAT3, 1:1000, Abcam, USA), anti-STAT3 antibody (1:1000, Proteintech, China), anti-LOX-1 antibody (1:1000, Proteintech, China), anti-FTO antibody (1:1000, Proteintech, China), anti-ALKBH5 antibody (1:1000, Proteintech, China), anti-RBM15 antibody (1:1000, Proteintech, China), anti-YTHDF1 antibody (1:1000, Proteintech, China), anti-YTHDF2 antibody (1:1000, Proteintech, China), anti-YTHDF3 antibody (1:1000, Proteintech, China), anti-IGF2BP1 antibody (1:1000, Proteintech, China), anti-IGF2BP2 antibody (1:1000, Proteintech, China), anti-IGF2BP3 antibody (1:1000, Proteintech, China), anti-EIF3A antibody (1:1000, Proteintech, China), anti-YTHDC1 antibody (1:1000, Proteintech, China), anti-YTHDC2 antibody (1:1000, Proteintech, China), and anti-GAPDH antibody (1:1000, Proteintech, China), the membranes were then incubated with peroxidase (HRP)-conjugated secondary antibody. .. After washes with PBS, protein bands Page 5/21 were detected using a chemiluminescence system (Bio-Rad, USA) and analyzed using Image Lab Software.

Immunohistochemistry:

Article Title: Downregulation of m 6 A Reader YTHDC2 Promotes the Proliferation and Migration of Malignant Lung Cells via CYLD/NF-κB Pathway
Article Snippet: .. IHC assay was used to analyze the expression of YTHDC2 in lung cancer and paired normal tissues with an anti-YTHDC2 antibody (1:500 dilusion; ProteinTech Group, Inc.). ..

Expressing:

Article Title: Downregulation of m 6 A Reader YTHDC2 Promotes the Proliferation and Migration of Malignant Lung Cells via CYLD/NF-κB Pathway
Article Snippet: .. IHC assay was used to analyze the expression of YTHDC2 in lung cancer and paired normal tissues with an anti-YTHDC2 antibody (1:500 dilusion; ProteinTech Group, Inc.). ..



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Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of <t>YTHDC2</t> , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.
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Image Search Results


Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

Journal: Frontiers in Veterinary Science

Article Title: METTL14 alleviates heat stress in Hu sheep involves enhancing fatty acid oxidation while reducing lipid deposition

doi: 10.3389/fvets.2025.1732947

Figure Lengend Snippet: Changes in expression of methylation-related genes and immunofluorescence assays with and without HS in primary hepatocytes. (A) Expression of METTL3 , METTL14, and WTAP in the RNA-seq of primary hepatocytes after HS; (B) Expression of FTO and ALKBH5 in the RNA-seq of primary hepatocytes after HS; (C) Expression of YTHDC2 , YTHDF2 and YTHDF3 in the RNA-seq of primary hepatocytes after HS; (D) Immunofluorescence assays of METTL14 and YTHDC2 with no HS and post HS. * p < 0.05, ** p < 0.01.

Article Snippet: Cultured hepatocytes were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.3% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) for 10 min, washed with PBS, and blocked with PBS containing 5% FBS and 0.3% TritonX-100 for 1 h. Thereafter, Primary antibodies against METTL14 (Proteintech, Rosemont, IL, USA) and YTHDC2 (Proteintech) were applied overnight at 4 °C.

Techniques: Expressing, Methylation, Immunofluorescence, RNA Sequencing